Fig. 5.

Effects of mutations in alternative helix #1 on processing and fidelity. (A) Primer extension analysis of RNA extracted from 30S subunits and 70S ribosomes from MC252 strain with a WT rrnB operon (pLK35) or with a rrnB operon producing 16S rRNA with deletion of 10 NTs upstream of the mature 5^′ end [p(Δ - 1 to  - 10)]. The lp16S rRNA in the MC252 strain transformed with p(Δ - 1 to  - 10) is shorter by 10 NTs than lp16S rRNA in the MC252 strain transformed with pLK35 and thus runs as a distinct product. (B) β-Galacosidase assay to monitor translational fidelity in MC252 containing p(Δ - 1 to  - 10) as described in Fig. 2 B.