Fig. 2.

(A) Anti-GPI IgG ASCs in different organs. Cells prepared from the indicated organs of 8-week-old K/BxN arthritic mice and BxN negative-control littermates were assayed by ELISPOT. The mean and SD are shown (n = 3). Cells were from peritoneal cavity exudates (PCE), bone marrow (BM), peripheral blood (PB), spleen (SP), and lymph nodes (LN). (B) Depletion of B cells and ASCs in SP and LNs of hCD20/K/g7 mice after rituximab treatment. Results from five independent experiments with the indicated lengths of drug administration to hCD20-positive and -negative littermates are shown. Each dot represents an individual mouse. The significance of the differences between data on hCD20/K/g7 (filled circles) and K/g7 (open circles) mice was calculated by one-way ANOVA using Stata.