Table 1.
| Table 1A. Proteins with known or putative function identified and assigned to mt membranes. They are grouped based on their association with demonstrated / predicted biological processes. The proteins were identified by indicated number of peptides (number of unique peptides in parenthesis) in TMM and MIM fractions. Blank columns indicate the proteins were not identified by MS in the analyzed samples. | |||||
|---|---|---|---|---|---|
| Protein_ID | GeneDB Description | TMD | GRAVY | TMM | MIM |
| Mitochondrial Respiration | |||||
| Complex III | |||||
| Tb09.211.4700 | RISP reiske iron-sulfur protein , mitochondrial precursor | 1 | −0.275 | 5(4) | 7(6) |
| Complex IV | |||||
| Tb11.01.3780 | cytochrome oxidase assembly factor, putative | 8 | 0.319 | 2(2) | |
| Tb09.160.1140 | electron transport protein SCO1/SCO2, putative | 1 | −0.489 | 1(1) | |
| Complex V | |||||
| Tb927.7.7420 | ATP synthase alpha chain, mitochondrial precursor | 0 | 0.082 | 11(11) | 11(11) |
| Tb927.3.1380 | ATP synthase beta chain, mitochondrial precursor | 0 | 0.083 | 9(9) | 10(10) |
| Other in aerobic respiration | |||||
| Tb927.7.5820 | monooxygenase, putative | 0 | 0.055 | 1(1) | |
| Electron transport | |||||
| Tb10.6k15.3640 | TAO alternative oxidase | 1 | −0.225 | 1(1) | 2(2) |
| Tb11.02.4485 | CYB5 cytochrome b5, putative | 1 | 0.071 | 1(1) | |
| Cation/Ion transport | |||||
| Tb09.160.2910 | tricarboxylate carrier, putative | 5 | 0.228 | 2(2) | 2(2) |
| Tb927.5.3400 | calcium-translocating P-type ATPase | 10 | 0.149 | 6(6) | 10(10) |
| Tb11.01.0720 | cation transporter, putative | 8 | 0.616 | 2(2) | |
| Amino acid transport | |||||
| Tb927.8.7600 | amino acid transporter, putative | 12 | 0.548 | 1(1) | 1(1) |
| Protein folding/modification/transport | |||||
| Tb11.01.4870 | inner membrane preprotein translocase Tim 17, putative | 2 | 1(1) | 1(1) | |
| Tb927.7.1340 | 10 kDa heat shock protein, putative | 0 | 0.181 | 2(2) | |
| Tb09.160.3090 | heat shock protein, putative | 1 | −0.388 | 2(2) | |
| Tb09.211.1550 | chaperone protein DNAJ, putative | 3 | −0.125 | 2(2) | 2(2) |
| Tb927.7.6200 | chaperone protein DNAJ, putative | 1 | −0.228 | 1(1) | |
| Tb11.03.0110 | chaperone protein DNAJ, putative | 1 | −0.559 | 1(1) | |
| Tb10.61.3100 | chaperone protein DNAJ, putative | 1 | −0.617 | 1(1) | 1(1) |
| Protein catabolic process | |||||
| Tb09.211.0680 | CAAX prenyl protease 1, putative | 5 | 0.051 | 2(2) | 1(1) |
| Tb11.01.6360 | metalloprotease, putative | 1 | −0.416 | 3(3) | 1(1) |
| Tb927.4.3300 | mitochondrial ATP-dependent zinc metallopeptidase, putative | 1 |
−0.194 |
1(1) |
3(3) |
| Tb927.4.4210 | ATP-dependent zinc metallopeptidase, putative | 2 | −0.319 | 3(3) | 2(2) |
| Tb09.211.0220 | rhomboid-like protein | 4 | 0.076 | 1(1) | 1(1) |
| Regulation | |||||
| Tb10.v4.0045 | prohibitin | 1 | −0.022 | 5(5) | 5(5) |
| Various Metabolic processes | |||||
| Tb927.3.1840 | 3-oxo-5-alpha-steroid 4-dehydrogenase, putative | 4 | 0.1 | 3(3) | 4(4) |
| Tb10.406.0290 | protein tyrosine phosphatase, putative | 7 | 0.613 | 1(1) | |
| Tb927.8.6410 | short-chain dehydrogenase, putative | 1 | −0.278 | 1(1) | |
| Tb927.5.1210 | short-chain dehydrogenase, putative | 2 | 0.156 | 4(4) | 5(5) |
| Tb10.6k15.3250 | succinyl-CoA ligase [GDP-forming] beta-chain, putative | 1 | −0.056 | 1(1) | |
| Tb927.6.4540 | 3-hydroxy-3-methylglutaryl-CoA reductase, putative | 1 | 0.086 | 1(1) | |
| Tb927.8.1420 | acyl-CoA dehydrogenase, mitochondrial precursor, putative | 0 | 0.167 | 1(1) | |
| Tb927.6.4210 | aldehyde dehydrogenase, putative | 0 | 0.144 | 3(3) | |
| Tb11.01.8470 | dihydrolipoyl dehydrogenase | 0 | 0.023 | 8(8) | 5(5) |
| Tb927.3.1790 | pyruvate dehydrogenase E1 beta subunit, putative | 0 | 0 | 7(7) | 6(6) |
| Tb10.70.4420 | methionine biosynthetic protein, putative | 0 | 0.123 | 1(1) | |
| Tb09.211.1960 | 3-demethylubiquinone-9 3-methyltransferase, putative | 0 | 0.089 | 1(1) | |
| Tb927.7.5680 | deoxyribose-phosphate aldolase, putative | 0 | 0.038 | 2(2) | |
| Tb10.70.5110 | mitochondrial malate dehydrogenase | 0 | 0.209 | 5(5) | 3(3) |
| Transport | |||||
| Tb09.211.1750 | mitochondrial carrier protein, putative | 1 | 0.079 | 6(6) | 5(5) |
| Tb10.389.0690 | mitochondrial carrier protein, putative | 4 | 0.144 | 1(1) | 2(2) |
| Tb10.61.1820 | mitochondrial carrier protein, putative | 3 | −0.021 | 4(4) | 5(5) |
| Tb11.02.2960 | mitochondrial carrier protein, putative | 1 | 0.297 | 2(2) | 2(2) |
| Tb09.211.2370 | mitochondrial carrier protein, putative | 0 | 0.27 | 1(1) | 1(1) |
| Tb927.2.2970 | mitochondrial carrier protein, putative | 0 | 0.173 | 3(3) | 1(1) |
| Tb927.3.2980 | mitochondrial carrier protein, putative | 0 | 0.161 | 1(1) | |
| Tb927.4.1660 | mitochondrial carrier protein, putative | 0 | 0.019 | 1(1) | |
| Tb11.03.0540 | ABC transporter, putative | 6 | 0.117 | 1(1) | |
| Table 1B. Proteins currently annotated as hypothetical assigned to mt membranes. | |||||
|---|---|---|---|---|---|
| Protein_ID | Domains/motifs | TMD | GRAVY | TMM | MIM |
| Complex IV | |||||
| Tb09.211.4740 | ubiE, ubiquinone/menaquinone biosynthesis methyltransferase | 1 | −0.511 | 3(3) | |
| Tb09.v1.0420 | 1 | −0.321 | 1(1) | ||
| Tb11.01.1900 | Macro domain, Appr-1-p processing | 1 | −0.127 | 1(1) | |
| Tb11.0400 | Tyrosine protein kinase | 1 | −0.227 | 2(2) | 2(2) |
|
Tb927.3.750 |
SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain; Putative methyl transferase |
1 |
−0.102 |
1(1) |
3(3) |
| Tb927.7.6990 | 1 | −0.347 | 1(1) | ||
| Tb11.01.5390 | DnaJ domain | 1 | −0.565 | 1(1) | |
| Tb11.02.5660 | 1 | −0.033 | 1(1) | 2(2) | |
| Complex V | |||||
| Tb10.70.7760 | LETM1, inner mitochondrial membrane protein | 1 | −0.628 | 2(2) | 2(2) |
| Tb11.47.0022 | 2 | −0.527 | 1(1) | 3(3) | |
| Tb927.7.840 | 1 | −0.231 | 1(1) | ||
| Tb11.02.4120 | 0 | 0.188 | 3(3) | 3(3) | |
| Novel Complex | |||||
| Tb927.8.580 | 1 | −0.25 | 2(2) | ||
| Other mt proteins | |||||
|
Tb10.6k15.1800 |
ABC1 family; ubiB, putative ubiquinone biosynthesis |
2 |
−0.017 |
1(1) |
1(1) |
| Tb10.26.0100 | 2 | 0.61 | 1(1) | ||
| Tb10.70.3010 | 1 | −0.49 | 3(3) | ||
| Tb10.70.4500 | 1 | −0.163 | 1(1) | ||
| Tb11.01.4740 | 1 | −0.251 | 1(1) | 1(1) | |
| Tb11.12.0014 | 2 | 0.191 | 2(2) | 1(1) | |
| Tb927.2.4090 | GTP-binding protein, HSR1-related | 2 | 0.055 | 1(1) | 1(1) |
| Tb927.2.4700 | 2 | −0.327 | 1(1) | 1(1) | |
| Tb927.5.510 | 1 | 0.098 | 1(1) | ||
| Tb927.7.640 | 3 | −0.06 | 2(2) | ||
| Tb927.8.5660 | 3 | 0.303 | 1(1) | ||