FIG. 1.

The decomposition of 13-HPODE with time. Linoleic acid was oxidized with soybean lipoxygenase, as described in Methods, and was incubated at 37°C for <72 h. At specified time periods, lipid peroxides and conjugated dienes were determined. (A) The determination of peroxides (▪) and conjugated diene (♦) during the decomposition of HPODE. The data are expressed as average ± SD from six different sets of incubations. (B, C) Determination of peroxides and conjugated dienes in the presence of α-tocopherol at 0 (♦), 5 (▪), 25 (▴), and 100 (×) μM concentrations. (D, E) Determination of peroxides and conjugated dienes in the presence of BHT at 0 (♦), 10 (▪), 50 (▴) and 100 (×) μM concentrations. The (B–E) values are too close, and the results are averages from four independent trials.