FIG. 2.

Decomposition of HPODE in serum. HPODE was prepared (∼30 μmols); an aliquot was set aside for peroxide, conjugated diene, and aldehyde determinations. Dried HPODE was resuspended in fetal calf serum at a concentration of 1.0 μmol in 100 μl and aliquoted into 96-well microtiter plates. Aliquots were also stored at −20°C. When needed, pyruvate was added in 5-μl quantities to give a final concentration of 5.0 μmols per 100 μl. Samples were collected at 1, 2, and 3 days after incubation at 37°C for analytic determinations. Incubations were set in quintuplicates, and the values represent average ± SD. *p < 0.05 versus sample incubated at 37°C. Open bars, grey bars, and black solid bars represent conjugated dienes, peroxides, and aldehydes, respectively.