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. 2010 Apr;51(4):709–719. doi: 10.1194/jlr.M001917

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Copyright © 2010 by the American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 2. — Purification of recombinant CGI-58. Recombinant His₁₂-CGI-58 was expressed in E. coli and purified over Ni²⁺-NTA-agarose. A: Coomassie Blue-stained SDS-PAGE gel showing molecular weight markers (lane 1), uninduced E. coli lysate (lane 2), isopropyl-β-D-1-thiogalactopyranoside-induced bacterial lysate (lane 3), supernatant following cell disruption by French press (lane 4), supernatant from the polyethyleneimine precipitation (lane 5), and eluant from Ni²⁺-NTA-agarose (lanes 6 and 7). B: Immunoblot of 3T3-L1 adipocyte lysate (lane 1) and eluant from Ni²⁺-NTA- agarose (lane 2) probed with polyclonal antiserum against mouse CGI-58.