Skip to main content
. 2010 Apr;51(4):832–842. doi: 10.1194/jlr.M002782

Fig. 5.

Fig. 5.

Glucose induces histone acetylation in CYP7A1 chromatin. A: HepG2 cells were infected by Ad-GFP or Ad-AMPKα2 at an MOI of ∼10 for 24 h followed by incubation in media supplemented with 5.5 mM or 27.5 mM glucose for additional 24 h. CYP7A1 mRNA levels were determined by real time PCR. * indicates statistical significance, n = 3, P < 0.05, 27.5 mM versus 5.5 mM glucose. B: HepG2 cells were treated with glucose (5.5 mM or 27.5 mM) for 24 h as indicated. The acetyl-H3 (Ac-H3) and acetyl-H4 (Ac-H4) in CYP7A1 gene chromatin were determined by ChIP assays as described under Materials and Methods. C: HepG2 cells were treated with vehicle control or AICAR (2 mM) for 24 h, and Ac-H3 and Ac-H4 occupancy in CYP7A1 chromatin was determined by ChIP assays. D: HepG2 cells were infected with Ad-GPF or Ad-AMPKα2 for 24 h. Ac-H3 and Ac-H4 in CYP7A1 chromatin were determined by ChIP assay.