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. 2010 Apr;51(4):832–842. doi: 10.1194/jlr.M002782

Fig. 6.

Fig. 6.

ACL is required for glucose induction of histone acetylation in CYP7A1. A: Immunoblot of ACL and AceCS1 in HepG2 cells transfected with siRNA control (sicon), siRNA to ACL (siACL) or siRNA to AceCS1 (siAceCS1). B: HepG2 cells were transfected with sicon, siACL, or siAceCS1 probe and treated with 5.5 mM or 27.5 mM glucose for 24 h as described under Materials and Methods. The mRNA expression levels were determined by real-time PCR. * indicates statistical significance, n = 3, P < 0.05, siACL versus sicon. C: HepG2 cells were transfected with sicon (open bar), siACL (gray bar), or siAceCS1 (closed bar), and cultured in 27.5 mM glucose for 24 h. ChIP assays were performed to detect Ac-H3 and Ac-H4 in CYP7A1 chromatin.