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. Author manuscript; available in PMC: 2011 Mar 10.
Published in final edited form as: Nano Lett. 2010 Mar 10;10(3):1022–1027. doi: 10.1021/nl904192m

Figure 3.

Figure 3

Acceptor fluorescence signal as a function of time (background subtracted). A signal peak is produced each time the donor-labeled helicase passes an acceptor dye on its encoder. For each event, the unwinding speed was determined by fitting a function of the form A cos2 [πf(t − t0)] to the data using least squares. One such fit is illustrated in (D). The speeds were (A) 759 ± 13 bp/s, (B) 950 ± 24 bp/s, (C) 665 ± 12 bp/s, and (D) 447 ± 6 bp/s. A complete event indicates that a single labeled helicase traveled at least 276 bp (A and B) or 345 bp (C and D). This implies processivity much greater than has previously been measured for DnaB alone.