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. 2010 Mar 22;5(3):e9776. doi: 10.1371/journal.pone.0009776

Figure 3. ArtinM and ConA also degranulate mesenteric mast cells in vitro only at high concentrations.

Figure 3

Mesenteries were incubated with 10 µg/ml ArtinM (A), 10 µg/ml ConA (B), 200 µg/ml ArtinM (C) or 200 µg/ml ConA (D). (Arrows, degranulated mast cells; *, non degranulated mast cells). Bar = 20 µm. Inset (C): Details of degranulated mast cell indicated by arrowhead. Bar = 10 µm Stain: Toluidine blue. Quantification of mesenteric mast cell degranulation by ArtinM or ConA (E). At 200 µg of either ArtinM or ConA there was significant (**p≤0.01) degranulation of mesenteric mast cells. The data shown is the average±SD of 3 separate experiments.