Figure 4. Long term treatment with PrioV3 or ICSM35 anti-prion antibodies abrogate PrP^Sc accumulation in ScN2a cells.

ScN2a cells were incubated for 4 days at 37°C (5% CO₂) with 25 µg ICSM35 or of PrioV3 and daily treatment renewal in tissue culture medium. BRIC126- and NCS-treated (Normal camel serum) were also included. The cells were then lysed and subsequently treated with PK prior to analysis of PrP^Sc levels by Western blot. Both PrioV3 and ICSM35 abrogated PrP^Sc replication when compared with the BRIC- and NCS-treated cells. Representative of three experiments.