Figure 6. PrioV3 but not ICSM35 anti-prion antibody permanently inhibits accumulation of PrP^Sc in ScN2a cells following cessation of antibody treatment.

ScN2a cells were incubated for 4 days at 37°C (5% CO₂) with 25 µg of (A) PrioV3 and (B) ICSM35* and daily treatment renewal in tissue culture medium. NCS- (Normal camel serum) and BRIC126-treated cells were also included. The cells were then further grown for another three with antibody-free tissue culture medium. The cells were then lysed and subsequently treated with PK prior to analysis of PrP^Sc levels by Western blot. PrioV3 treatment led to permanent inhibition of PrP^Sc replication but ICSM35-treated cultures displayed de-novo replication of PrP^Sc; both the BRIC- and NCS-treated cells showed high levels of PrP^Sc. Representative of three experiments.