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. 2010 Mar 22;5(3):e9808. doi: 10.1371/journal.pone.0009808

Figure 6. Endo180 mediates invasion of glioblastoma cells through a collagen matrix.

Figure 6

(A) SF188 cells were treated with control or Endo180 siRNA oligonucleotides. After 48 h, cells were plated on Transwell inserts coated with collagen I and allowed to migrate towards a high serum concentration for 24 h. Data represents >6 independent experiments. Parallel cultures were subject to immunoblotting to confirm siRNA mediated Endo180 downregulation. (B) Growth of SF188 cells stably infected with control or Endo180 shRNA lentiviruses was monitored over 4 days using Cell Titre-Glo and subject to immunoblotting to confirm shRNA mediated Endo180 downregulation. Error bars represent mean values from two independent experiments each with triplicate samples ±SEM. (C) Control and Endo180 shRNA infected SF188 cells were plated onto a thick collagen I-containing gel and cultured for 11 days. Gels were embedded, sectioned, immunostained with vimentin and an invasion index calculated. Left hand panel shows representative images. Arrows indicate depth of matrix. Right hand panel shows invasion index from 4 independent experiments each with duplicate or triplicate samples. p-values were generated using the student's two-tailed t-test.