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. Author manuscript; available in PMC: 2011 Mar 19.
Published in final edited form as: Circ Res. 2010 Jan 28;106(5):932–940. doi: 10.1161/CIRCRESAHA.109.207415

Figure 4. Endothelial cell proliferation and tube formation assays.

Figure 4

(A) Proliferation assay and (B) tube formation assay with endothelial cells isolated from Adam17flox/flox/Tie2-Cre mice or Adam17flox/flox controls (see materials and methods). There was no significant difference in proliferation at day 4 (d4) of the culture plated a comparable density on day 0 (d0) (A), but a clear decrease in tube formation was seen in endothelial cells from Adam17flox/flox/Tie2-Cre mice compared to controls (B), which could be largely rescued by treatment with 5ng/ml HB-EGF, but not with 5ng/ml VEGF-A.