The expression of Errfi1 by real-time RT-PCR and in situ hybridization in pseudopregnancy. A) The expression level of Errfi1 was measured in uteri of pseudopregnancy. Total RNA used for the RT-PCR assays was prepared from pseudopregnant uteri. The results represent the mean ± SEM of three independent RNA sets. **P < 0.01 and ***P < 0.001. B) The localization pattern of Errfi1 mRNA by in situ hybridization during early pregnancy. Nuclei are counterstained with hematoxylin. E, embryonic day; EM, embryo; LE, luminal epithelium; GE, glandular epithelium; PDZ, primary decidual zone; SDZ, secondary decidual zone; ST, stroma cells; MYO, myometrium; bar = 500 μm. Arrowheads indicate luminal and glandular epithelium.