FIG. 4.

Effect of BADGP on RL2 immunoreactivity in embryos cultured under conditions that elevate levels of RL2 immunoreactivity. Zygotes (16–18 h post-hCG) were cultured in the presence and absence of 0.2 mM glucose (Glu) or 0.2 mM glucosamine (GlcN) and in the presence and absence of 2 mM BADGP. DMSO in KSOM at concentration equivalent to 2 mM BADGP was also included as solvent control. At 68 h post-hCG, the effect of these treatments on nuclear levels of RL2 immunoreactivity was assessed as previously described in each of two experiments each with at least five embryos per treatment. Each bar represents the mean (± SEM) nuclear immunoreactivity per embryo. Factorial ANOVA determined no experimental variation and no interaction. Means with the same lowercase letters are significantly different (c: P < 0.01; a, b, d–h: P < 0.001; Tukey post hoc test). Representative confocal immunofluorescent images of embryos in each treatment are also presented above each bar. Original magnification ×250; bar = 25 μm.