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. 2009 Dec 23;82(4):751–758. doi: 10.1095/biolreprod.109.076661

FIG. 6.

FIG. 6.

Effects of culture treatment on cell number at 90 h post-hCG. Zygotes were cultured from 18 to 90 h post-hCG in control KSOM (KSOM), KSOM supplemented with 27 mM glucose (++Glu) , KSOM with 27 mM glucose supplemented with 2 mM BADGP (++Glu/BADGP), KSOM supplemented with 26.8 mM sucrose as osmolarity control (Sucrose), 0.2 mM GlcN (GlcN), 0.2 mM GlcN supplemented with 2 mM BADGP (GlcN/BADGP), KSOM with DMSO equivalent to 2 mM BADGP (DMSO), and KSOM supplemented with 2 mM BADGP (BADGP). Embryos were fixed at 90 h post-hCG, and cell nuclei were stained with propidium iodide. Bars represent mean number of cells per embryo ± SEM from three separate experiments each with 30 zygotes per treatment. Two-way ANOVA determined no interexperimental variation (P > 0.05). Means were further analyzed to determine differences between different treatments. Means with the same lowercase letters are statistically different (a–c, e, f, h–t: P < 0.001; d, g: P < 0.05; Tukey post hoc test).