Table 1.
Inhibitor profile of fibrinopeptide release by L-ficolin mannan-binding lectin-associated serine proteases (MASPs) bound to CysNAc-Sepharose from normal human serum
| Inhibitor (concentration) | % inhibition of fibrinopeptide release |
|---|---|
| C1 inhibitor (4 μg/ml) | 90 |
| Hirudin (1·75 μg/ml) | 80 |
| EDTA (10 mm)1 | 90 |
| Soybean trypsin inhibitor (10 μg/ml) | 0 |
| Pefabloc SC (1 mm) | 95 |
| Anti-thrombin III (2 μg/ml) | 60 |
| Anti-thrombin + heparin (2 μg/ml + 55 μg/ml) | 90 |
| α2-macroglobulin (25 ng/ml) | 55 |
1
Ethylenediaminetetraacetic acid (EDTA) was added to serum to a final concentration of 10 mm and a washing buffer containing 10 mm EDTA was used. For the other inhibitors.