Figure 4.

The effect of immunization on A3G activity; correlation with decreased viral replication and increased viral neutralization and in vitro effect of cytoskeletal proteins on A3G activity. (a) Apolipoprotein B mRNA-editing enzyme-catalytic polypeptide-like 3G (APOBEC3G) assayed by real-time polymerase chain reaction (PCR) in peripheral blood mononuclear cells (PBMCs) before and after immunization of macaques with ATP-heat-shock protein (HSP) (•), compared with PBMCs from ADP-HSP (○). (b) Correlation between APOBEC3G mRNA and reverse transcriptase of PBMCs from post-ATP-HSP- (•) and post-ADP-HSP (○)-immunized macaques. (c) Correlation between serum neutralizing activity and APOBEC3G mRNA. (d) In vitro stimulation with cofilin (•), profilin (○) and β/γ-actin (Δ) of PBMCs from unimmunized, ATP-HSP-immunized and ADP-HSP-immunized macaques; APOBEC3G-fold increase was calculated relative to that of unstimulated cells.