Table 1.

Characterization of proteins fractionated by two-dimensional polyacrylamide gel electrophoresis (PAGE) and identified by MALDI-Tof/Tof mass spectrometry

Spot no.	Protein name	Accession number	Theoretical pI	Theoretical MW	Protein score	Sequence coverage (%)	Number of matched peptides	Precursor ion mass	Peptide sequence	Ion score
ATP 1	DnaK-Hu	A27077	5·37	70 854·2	565	45·66	26	1981·9978	TVTWAWTVPAYFNDSQR	129
2	β-Actin	Q96HG5	5·78	40 477·2	608	58·9	16	1790·8918	SYELPDGQVITGNER	122
3	NDPK	A33386	5·83	17 137·7	354	50·65	11	1344·7634	TFIAIKPDGVQR	84
4	NDPK	A33386	5·83	17 137·7	344	70·39	12	1149·6415	DRPFFAGLVK	79
5	NDPK	1NUEA	8·55	17 155·9	368	74·8	12	1344·7634	TFIAIKPDGVQR	100
6	NDPK	1NUEA	8·55	17 155·9	305	78·14	12	1175·6571	DRPFFPGLVK	50
ADP 7	DnaK-Hu	A27077	5·37	70 854·2	397	48·29	26	2774·3267	QTQFTTYSDNA QPGVLIQVYEGER	34
8	HSC-1	Q961S6	5·36	64 562·1	273	26·92	15	1081·5676	LLQDFFNGK	54
9	HSC-1	Q961S6	5·36	64 562·1	331	23·67	14	1253·6161	FEELNADLFR	72
10	HSC-1	Q961S6	5·36	64 562·1	335	33·73	16	1253·6161	FEELNADLFR	72
11	HSP70-2	AAD21815	5·48	69 982	182	24·02	15	1109·5789	LLQDFFNGR	29
12	γ-Actin	P63261	5·55	40 194·1	431	54·7	17	1516·7026	QEYDESGPSIVHR	94
13	HP (actin family)	Q8WVW5	5·78	40 477·2	367	48·7	12	1790·8916	SYELPDGQTIVIGNER	90
14	HP (actin family)	Q8WVW5	5·78	40 477·2	210	22·8	6	1790·8916	SYELPDGQTIVIGNER	83
15	Pyridoxal kinase	BAA95540	5·75	35 079·9	115	29·5	10	–	–
16	Pyridoxal kinase	BAA95540	5·75	35 079·9	390	48·29	16	2282·289	VVPLADIITPNQFEAELLSGR	73
17	Adenosine kinase	G02049	6·09	37 488	370	32·6	14	1171·6582	AGHYAASIIIR	80
18	HSC-54	BAB18615	5·62	53 484	446	37·93	19	1981·9978	TVTNAVVTVPAYFNDSQR	88
19	DnaK-Hu	A27077	5·37	70 854·2	356	30·03	20	1961·9976	TVTNAVVTVPAYFNDSQR	70
20	HSC-54	BAB18615	5·62	53 484·4	308	33·26	15	1961·9978	TVTNAVVTVPAYFNDSQR	42
21	HSnm23	CAA35621	7·07	20 398·3	226	63·33	12	1344·7634	TFIAIKPDGVOR	45
22	NDPK	A33386	5·83	17 137·6	162	70·39	12	1149·6415	DRPFFAGLVK	11
23	NDPK	A33386	5·83	17 137·6	119	56·57	8	1149·6415	DRPFFAGLVK	18
24	NDPK	A33386	5·83	17 137·6	151	56·77	9	1149·6415	DRPFFAGLVK	17
26	NDPK	1NSKR	8·87	17 157·9	70	50·33	6	1175·6571	DRPFFPGLVK	5
28	NDPK	AAC05177	8·76	15 519	116	54·0	7	1175·6571	DRPFFPGLVK	29
29	NDPK	AAC05177	8·76	15 519	84	42·34	6	1175·6571	DRPFFPGLVK	17
30	NDPK	1NSKR	8·87	17 157·9	136	62·5	8	1175·6571	DRPFFPGLVK	37
31	PKC-1 inhibitor	HINT_HUMAN	6·46	13 662	97	54·76	5	1416·7594	AQVARPGGDTIFGK	19
32	NDPK	QNSKR	8·55	1715·9	158	62·91	8	1801·9113	VMLGETNPADSKPGTR	43
33	NDPK	AAC05177	8·76	15 519	142	54·01	7	1175·6571	DRPFFPGLVK	41
34	NDPK	AAC05177	8·76	15 519	131	67·88	8	1175·6571	DRPFFPGLVK	30
35	Profilin	1AWIA	8·46	14 842·8	163	65·94	9	1470·7587	SSFYVNGLTLGGQK	50
36	Cofilin	S12632	8·22	18 490·7	226	52·41	8	2166·0964	EILVGDGQTVDDPYATFVK	68
37	NDPK	1NUEA	8·55	17 155·9	186	64·23	11	1344·7634	TFIAIKPDGVQR	42
38	Cofilin	S12632	8·22	18 490·7	147	46·98	7	1990·0684	KEDLVFIFWAPESAPLK	38
39	NDPK	1NSKR	8·87	17 157·9	143	77·63	12	−1175·6571	DRPFFPGLVK	12
40	Profilin	A28622	8·44	15 044·6	154	92·02	11	1470·7587	DRPFFPGLVK	18

The protein name and accession number represent the candidate proteins identified with the highest significant score from the mass spectrometry database (MSDB) by the Mascot search engine. The protein score is defined as −10 × log(P), where P is the probability that the observed match is a random event and protein scores greater than 64 are significant (P < 0·05). Non-significant scores are not shown. The theoretical isoelectric point (pI) and molecular weight (MW, Daltons) for each identified protein are shown. Where tandem mass spectrometry (MSMS) was performed, the precursor ion mass and resulting amino acid sequences are shown together with the corresponding ion score. Only the highest ion score and its corresponding peptide are shown.

HP, hypothetical protein; NDPK, nucleoside diphosphate kinase; HSC, heat shock protein cognate-1; PKC, protein kinase c; HSnm23, Homo sapiens NPDK like protein.