Table 2.
Serum immunoglobulin G (IgG) antibodies to heat-shock protein 70 (HSP70), cofilin and profilin pre- and post-immunization with HSP-ADP or HSP-ATP, assayed by enzyme-linked immunosorbent assay (ELISA)
ADP-HSP |
ATP-HSP |
|||||||
---|---|---|---|---|---|---|---|---|
Pre | Post | t | P | Pre | Post | t | P | |
HSP-70 | 2·2 (0·2) | 2·4 (0·5) | 0·29 | 0·783 | 2·0 (0·2) | 2·6 (0·3) | 1·47 | 0·20 |
γ-Actin | 0·7 (0·24) | 3·1 (0·35) | 3·36 | 0·078 | 2·3 (0·58) | 3·0 (0·14) | 1·11 | 0·383 |
β-Actin | 1·5 (0·13) | 2·7 (0·24) | 3·93 | 0·059 | 1·7 (0·36) | 3·2 (0·25) | 9·5 | 0·010 |
Cofilin | 2·8 (0·7) | 5·8 (0·7) | 4·74 | 0·009 | 3·0 (0·4) | 3·8 (0·2) | 1·57 | 0·22 |
Profilin | 3·6 (0·9) | 5·2 (0·6) | 3·14 | 0·035 | 3·5 (0·3) | 3·5 (0·6) | 0·00 | 1·0 |
Values are mean (± standard error of the mean) log2 titres; the t and P values were determined by paired Student’s t-test.