Figure 5.

Levels of interleukin (IL)-12p70 and IL-10 were determined in supernatants of resveratrol-treated dendritic cells (DCs) and controls after 48 hr of lipopolysaccharide (LPS) stimulation. DCs were prepared as described in the Materials and methods and treated with various doses of resveratrol (from 3 to 50 μm) from t = 0. Even at low doses, resveratrol markedly inhibited the ability of DCs to produce IL-12p70, and completely inhibited LPS-induced IL-12p70 production at 50 μm. Simultaneously with dose-dependent inhibition of IL-12p70, treated DCs dose-dependently produced more IL-10. Data presented are the mean ± standard deviation of three independent experiments.