Figure 3.

CCL19 is internalized by chemokine receptor on activated macrophages B (CRAM-B) expressing CHO cells. CCL19 binding was assessed in cells shifted to 37° compared with 4° before a washing step in acidic buffer. Data show the relative increased association of ¹²⁵I-labelled CCL19 to CRAM-B-transfected CHO-K1 when compared with mock-transfected cells in the same experimental conditions. Resistance to acid-mediated removal of bound chemokine in CRAM-B-transfected cells occurs after allowing internalization of the chemokine at 37°. The figure shows mean and SEM of three independent experiments realized in triplicate.