Figure 4.
Cytokine production by splenocytes in response to Chlamydia muridarum stimulation. Groups of nine mice were inoculated intranasally with 2000 inclusion-forming units (IFU) of C. muridarum and splenocytes were prepared at day 12 followed by stimulation with or without heat-killed C. muridarum elementary bodies (EBs) for 72 hr. The level of C. muridarum responsive interferon-γ (IFN-γ) and interleukin-17 (IL-17) in splenocyte culture supernatants was analysed by enzyme-linked immunosorbent assay. The data represent the mean ± SE from nine individual mice. *P < 0·05.
