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. 2010 Apr;129(4):578–588. doi: 10.1111/j.1365-2567.2009.03160.x

Figure 3.

Figure 3

Suppression of innate immune responses by 4-hydroxy-2-heptylquinoline (HHQ) and 2-heptyl-3,4-dihydroxyquinoline (PQS, Pseudomonas quinolone signal). (a,b) J774A.1 cells were treated with extracts of culture supernatant from the pqsA mutant at 1/16× concentration for 24 hr in the presence or absence of 0·28 μg/ml of HHQ or PQS. The amount of tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from the culture supernatants was measured by enzyme-linked immunosorbent assay. Data represent mean ± SD (n = 8). Statistical differences among groups were analysed by analysis of variance. The dimethyl sulphoxide vehicle control showed no difference compared with no-treatment control in Fig. 2 so it was not included in the experiment shown here. (c) Cell viability after treatment with various concentrations of HHQ or PQS for 24 hr was measured by MTS assay. Data represent mean ± SD (n = 4). All data (a–c) are representative of three independent experiments with similar results.