Figure 2.

p21^Cip1 induced by n-butyrate was maintained in anergic T helper type 1 (Th1) cells. The Th1 cells were stimulated with keyhole limpet haemocyanin (day 0). n-Butyrate was added to some of the Th1 cells on day 1 (D₁). Total cell lysates were prepared daily during the 6-day primary culture and Western blot analysis of p21^Cip1 (a) and other cell-cycle proteins (b) was performed. Because n-butyrate was added to the cultures at the end of day 1, there is a common day 1 (D₁) lane for the two groups. As there was insufficient material, the D₁ level of some proteins could not be included. Densitometric analysis of the p21^Cip1 levels was performed and the are results presented as per cent adjusted volume (PAV). Day 6 levels of p21^Cip1 were studied in four separate experiments with consistent results.