Figure 6.

p21^Cip1 interacted with phosphorylated c-Jun N-terminal kinase (p-JNK) and p-c-jun in anergic T helper type 1 (Th1) cells in reciprocal immunoprecipitations. Th1 cells from primary cultures stimulated with antigen in the absence (lane 1) or presence (lane 2) of n-butyrate were restimulated with anti-CD3 and anti-CD28 antibody-coated beads for 2 hr before lysis. p-JNK (a) and p-c-jun (b) were immunoprecipitated from lysates, separated by sodium dodecyl sulphate–polyacrylamide gel electrophoresis and then immunoblotted with antibodies specific for p21^Cip1, p27^Kip1and p-JNK.