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. 2010 Apr;129(4):589–599. doi: 10.1111/j.1365-2567.2009.03161.x

Figure 7.

Figure 7

c-Fos and c-jun activity was inhibited in the anergic T helper type 1 (Th1) cells. (a) Th1 cells were stimulated with antigen ± n-butyrate in primary cultures. The Th1 cells were then restimulated using anti-CD3 and anti-CD28 antibody-coated beads for 2 hr for c-fos assay and 1 hr for c-jun assay. Nuclear lysates were prepared from restimulated Th1 cells as well as from unstimulated resting Th1 cells (group R). c-fos and c-jun activity was measured using TransAM Transcription Factor Activity Assay kits. This experiment was performed twice with consistent results. Statistical differences between control and anergic Th1 cells are indicated as **P< 0·01 or *P < 0·05. (b) Th1 cells were stimulated with immobilized anti-CD3 and anti-CD28 antibodies in the presence or absence of 5 μm SP600125 for 24 hr. Proliferation was measured by [3H]thymidine incorporation. This experiment has been repeated twice with similar results. Asterisk indicates statistical difference differences between Th1 cells incubated in the presence or absence of SP600125.