Figure 2.

Flow cytometric analysis of [Nle⁴, DPhe⁷]-α-melanocyte-stimulating hormone (NDP-MSH) treated and untreated mature and immature dendritic cells (DCs). The DCs were generated in vitro as described in the Materials and methods section in the presence or absence of NDP-MSH. DCs were treated with lipopolysaccharide (LPS; 1 μg/ml) for 40 hr to generate mature DCs. DCs were then incubated with major histocompatibility complex (MHC) Class I (HLA-A,B,C), MHC Class II (HLA-DR), CD86 and CD83 antibodies and were analysed by flow cytometry. NDP-MSH does not influence markers associated with DC maturation, with the exception of CD86 which is down-regulated in NDP-MSH-treated mature DCs. Filled histograms (control), thin lines (DCs in media) and thick lines (DCs treated with NDP-MSH). Data are representative of three separate experiments.