Figure 4.

Flow cytometric analysis of endocytic activity of immature dendritic cells (DCs) pretreated with [Nle⁴, DPhe⁷]-α-melanocyte-stimulating hormone (NDP-MSH). Filled histograms show the uptake of fluorescein isothiocyanate (FITC) -dextran at 37° by immature DCs cultured in medium alone. Overlay represents the uptake of immature DCs treated with NDP-MSH in parallel experiments. Endocytic activity was also measured at 4° (0 min sample) to control for non-specific fluorescence. NDP-MSH does not affect DC endocytosis. Data are representative of three independent experiments.