Table 1.

Melanocortin-receptor-specific primers

Primer (5′ to 3′)	Sequence	Expected PCR product size (bp)
MC-1R upper	GCCACCATCGCCAAGAACCG	416
MC-1R lower	ATAGCCAGGAAGAAGACCAC
MC-2R upper	GAAGCACATTATCAACTCGTATG	539
MC-2R lower	GCGACGTGAAGGTGATCAGTCTG
MC-3R upper	GAGCAGCAGCGCCTTCTGTG	462
MC-3R lower	CGAGTAGACGATGAACACCAC
MC-4R upper	CAATAGCCAAGAACAAGAATC	564
MC-4R lower	GACAACAAAGACGCCAATCAG (see ref. 13)
MC-5R upper	CATTGCTGTGGAGGTGTTTCT	425
MC-5R lower	GTAGGTGGATTCTGAGTACAG (see ref. 13)

bp, base pairs; PCR, polymerase chain reaction.