Table 4.
Statistically significant changed gene ontology at each time point
| Time point (h) |
Up-regulation |
Down-regulation |
||||||||
| GO ID | GO name | Genes | P value | Code | GO ID | GO name | Genes | P value | Code | |
| 0.5 | GO:0008201 | Heparin binding | 5 | 7.1E-4 | MF | GO:0006955 | Immune response | 20 | 0.00000 | BP |
| GO:0008134 | Transcription factor binding | 4 | 0.01585 | MF | GO:0009615 | Response to virus | 10 | 0.00000 | BP | |
| GO:0003700 | Transcription activity | 10 | 0.02835 | MF | GO:0008150 | Biological process | 15 | 0.00896 | BP | |
| GO:0008083 | Growth factor activit | 4 | 0.03882 | MF | GO:0007267 | Cell-cell signaling | 10 | 0.00966 | BP | |
| GO:0005576 | Extracellular region | 15 | 0.02875 | CC | GO:0006935 | Chemotaxis | 6 | 0.01581 | BP | |
| GO:0005634 | Nucleus | 28 | 0.03452 | CC | GO:0006954 | Inflammatory response | 8 | 0.01581 | BP | |
| GO:0008285 | Negative regulation of cell proliferation | 7 | 0.03430 | BP | ||||||
| GO:0007275 | Multicellular organismal development | 16 | 0.03576 | BP | ||||||
| GO:0008009 | Chemokine activity | 7 | 0.00000 | MF | ||||||
| GO:0046870 | Cadmium ion binding | 3 | 0.00194 | MF | ||||||
| GO:0016779 | Nucleotidyl transferase activity | 5 | 0.02486 | MF | ||||||
| GO:0005576 | Extracellular region | 37 | 0.00000 | CC | ||||||
| GO:0005615 | Extracellular space | 14 | 2.0E-4 | CC | ||||||
| GO:0005634 | Nucleus | 56 | 7.0E-4 | CC | ||||||
| 1 | GO:0008201 | Heparin binding | 5 | 0.00265 | MF | GO:0008009 | Chemokine activity | 6 | 3.5E-4 | MF |
| GO:0003700 | Transcription factor activity | 13 | 0.00886 | MF | GO:0046870 | Cadmium ion binding | 3 | 0.00264 | MF | |
| GO:0005515 | Protein binding | 38 | 0.01716 | MF | GO:0003677 | DNA binding | 26 | 0.00264 | MF | |
| GO:0045766 | Positive regulation of angiogenesis | 3 | 0.01125 | BP | GO:0046872 | Metal ion binding | 36 | 0.01144 | MF | |
| GO:0001558 | Regulation of cell growth | 6 | 0.01502 | BP | GO:0008270 | Zinc ion binding | 34 | 0.02041 | MF | |
| GO:0006915 | Apoptosis | 8 | 0.02591 | BP | GO:0003674 | Molecular function | 15 | 0.02257 | MF | |
| GO:0008285 | Negative regulation of cell proliferation | 6 | 0.02591 | BP | GO:0003676 | Nucleic acid binding | 13 | 0.02257 | MF | |
| GO:0005634 | Nucleus | 36 | 0.00597 | CC | GO:0016779 | Nucleotidyl transferase activity | 4 | 0.02571 | MF | |
| GO:0005575 | Cellular component | 10 | 0.02160 | CC | GO:0005515 | Protein binding | 61 | 0.03204 | MF | |
| GO:0003704 | Specific RNA polymerase II transcription factor activity | 3 | 0.04080 | MF | ||||||
| GO:0009615 | Response to virus | 10 | 0.00000 | BP | ||||||
| GO:0006955 | Immune response | 18 | 0.00000 | BP | ||||||
| GO:0006355 | Regulation of transcription DNA-dependent | 39 | 4.0E-5 | BP | ||||||
| GO:0006350 | Transcription | 31 | 4.5E-4 | BP | ||||||
| GO:0008150 | Biological process | 18 | 0.00348 | BP | ||||||
| GO:0007267 | Cell-cell signaling | 11 | 0.00480 | BP | ||||||
| GO:0006954 | Inflammatory response | 8 | 0.03385 | BP | ||||||
| GO:0045087 | Innate immune response | 5 | 0.04274 | BP | ||||||
| GO:0005634 | Nucleus | 71 | 0.00000 | CC | ||||||
| GO:0005576 | Extracellular region | 37 | 1.1E-4 | CC | ||||||
| GO:0005615 | Extracellular space | 13 | 0.00474 | CC | ||||||
| GO:0005622 | Intracellular | 31 | 0.01344 | CC | ||||||
| GO:0005575 | Cellular component | 15 | 0.03381 | CC | ||||||
| 2 | GO:0003700 | Transcription factor activity | 18 | 1.4E-4 | MF | GO:0009615 | Response to virus | 10 | 0.00000 | BP |
| GO:0008201 | Heparin binding | 5 | 0.00193 | MF | GO:0006955 | Immune response | 16 | 0.00000 | BP | |
| GO:0043565 | Sequence-specific DNA binding | 10 | 0.01819 | MF | GO:0008150 | Biological process | 18 | 6.6E-4 | BP | |
| GO:0008083 | Growth factor activity | 5 | 0.01885 | MF | GO:0007267 | Cell-cell signaling | 10 | 0.01111 | BP | |
| GO:0005178 | Integrin binding | 3 | 0.02722 | MF | GO:0006954 | Inflammatory response | 8 | 0.01911 | BP | |
| GO:0008134 | Transcription factor binding | 4 | 0.02722 | MF | GO:0045087 | Innate immune response | 5 | 0.02866 | BP | |
| GO:0008009 | Chemokine activity | 3 | 0.02849 | MF | GO:0007565 | Female pregnancy | 5 | 0.03928 | BP | |
| GO:0046872 | Metal ion binding | 23 | 0.04806 | MF | GO:0005576 | Extracellular region | 36 | 1.0E-5 | CC | |
| GO:0045944 | Positive regulation of transcription from RNA polymerase II promoter | 7 | 0.00234 | BP | GO:0005615 | Extracellular space | 13 | 0.00113 | CC | |
| GO:0006955 | Immune response | 10 | 0.00470 | BP | GO:0005634 | Nucleus | 51 | 0.00899 | CC | |
| GO:0008285 | Negative regulation of cell proliferation | 7 | 0.00681 | BP | GO:0046870 | Cadmium ion binding | 3 | 0.01145 | MF | |
| GO:0000122 | Negative regulation of transcription from RNA polymerase II promoter | 6 | 0.00681 | BP | GO:0016831 | Carboxy-lyase activity | 3 | 0.02198 | MF | |
| GO:0006915 | Apoptosis | 9 | 0.00713 | BP | GO:0030674 | Protein binding bridging | 4 | 0.04373 | MF | |
| GO:0006954 | Inflammatory response | 7 | 0.00769 | BP | ||||||
| GO:0001558 | Regulation of cell growth | 5 | 0.00914 | BP | ||||||
| GO:0009611 | Response to wounding | 3 | 0.01457 | BP | ||||||
| GO:0005615 | Extracellular space | 12 | 8E-5 | CC | ||||||
| GO:0005634 | Nucleus | 42 | 2.4E-4 | CC | ||||||
| GO:0005576 | Extracellular region | 22 | 4E-4 | CC | ||||||
| GO:0030173 | Integral to Golgi membrane | 3 | 0.02101 | CC | ||||||
| 4 | GO:0008083 | Growth factor activity | 8 | 1.0E-5 | MF | GO:0009615 | Response to virus | 12 | 0.00000 | BP |
| GO:0005125 | Cytokine activity | 6 | 3.7E-4 | MF | GO:0007565 | Female pregnancy | 9 | 1.6E-4 | BP | |
| GO:0046983 | Protein dimerization activity | 6 | 0.00123 | MF | GO:0006955 | Immune response | 17 | 5.0E-4 | BP | |
| GO:0005100 | Rho GTPase activator activity | 3 | 0.00268 | MF | GO:0001525 | Angiogenesis | 7 | 0.02671 | BP | |
| GO:0008201 | Heparin binding | 4 | 0.00826 | MF | GO:0007267 | Cell-cell signaling | 10 | 0.02671 | BP | |
| GO:0003700 | Transcription factor activity | 13 | 0.00826 | MF | GO:0008150 | Biological process | 19 | 0.02928 | BP | |
| GO:0008047 | Enzyme activator activity | 3 | 0.01045 | MF | GO:0016477 | Cell migration | 5 | 0.03984 | BP | |
| GO:0005178 | Integrin binding | 3 | 0.01447 | MF | GO:0005576 | Extracellular region | 45 | 0.00000 | CC | |
| GO:0016563 | Transcription activator activity | 4 | 0.02237 | MF | GO:0005577 | Fibrinogen complex | 3 | 6.0E-4 | CC | |
| GO:0005515 | Protein binding | 33 | 0.03960 | MF | GO:0005615 | Extracellular space | 14 | 0.00843 | CC | |
| GO:0043565 | Sequence-specific DNA binding | 8 | 0.03960 | MF | GO:0031093 | Platelet α granule lumen | 4 | 0.01203 | CC | |
| GO:0006955 | Immune response | 11 | 2.4E-4 | BP | GO:0016020 | Membrane | 61 | 0.03962 | CC | |
| GO:0006915 | Apoptosis | 9 | 0.00440 | BP | GO:0005794 | Golgi apparatus | 15 | 0.03962 | CC | |
| GO:0030183 | B cell differentiation | 3 | 0.01798 | BP | ||||||
| GO:0045944 | Positive regulation of transcription from RNA polymerase II promoter | 5 | 0.03323 | BP | ||||||
| GO:0000079 | Regulation of cyclin-dependent protein kinase activity | 3 | 0.03704 | BP | ||||||
| GO:0007050 | Cell cycle arrest | 4 | 0.03704 | BP | ||||||
| GO:0008284 | Positive regulation of cell proliferation | 5 | 0.03704 | BP | ||||||
| GO:0007267 | Cell-cell signaling | 6 | 0.03704 | BP | ||||||
| GO:0001558 | Regulation of cell growth | 5 | 0.0407 | BP | ||||||
| 6 | GO:0005515 | Protein binding | 65 | 0.00000 | MF | GO:0046870 | Cadmium ion binding | 3 | 0.00135 | MF |
| GO:0003700 | Transcription factor activity | 24 | 1.0E-5 | MF | GO:0003674 | Molecular function | 13 | 0.00852 | MF | |
| GO:0008083 | Growth factor activity | 8 | 3.5E-4 | MF | GO:0008009 | Chemokine activity | 4 | 0.00852 | MF | |
| GO:0003714 | Transcription co-repressor activity | 7 | 3.5E-4 | MF | GO:0003950 | NAD+ADP-ribosyl transferase activity | 3 | 0.01169 | MF | |
| GO:0005125 | Cytokine activity | 8 | 3.5E-4 | MF | GO:0030674 | Protein binding bridging | 3 | 0.04041 | MF | |
| GO:0005100 | Rho GTPase activator activity | 4 | 3.5E-4 | MF | GO:0009615 | Response to virus | 12 | 0.00000 | BP | |
| GO:0003700 | Transcription factor activity | 7 | 6.2E-4 | MF | GO:0006955 | Immune response | 16 | 0.00000 | BP | |
| GO:0046983 | Protein dimerization activity | 7 | 6.9E-4 | MF | GO:0007565 | Female pregnancy | 9 | 0.00000 | BP | |
| GO:0008270 | Zinc ion binding | 32 | 0.00504 | MF | GO:0008150 | Biological process | 14 | 0.00612 | BP | |
| GO:0046872 | Metal ion binding | 32 | 0.00827 | MF | GO:0007267 | Cell-cell signaling | 8 | 0.00676 | BP | |
| GO:0005085 | Guanyl-nucleotide exchange factor activity | 5 | 0.02023 | MF | GO:0006952 | Defense response | 5 | 0.00728 | BP | |
| GO:0043565 | Sequence-specific DNA binding | 11 | 0.03272 | MF | GO:0030168 | Platelet activation | 3 | 0.01864 | BP | |
| GO:0008201 | Heparin binding | 4 | 0.03502 | MF | GO:0051258 | Protein polymerization | 3 | 0.03966 | BP | |
| GO:0005178 | Integrin binding | 3 | 0.04652 | MF | GO:0005576 | Extracellular region | 44 | 0.00000 | CC | |
| GO:0006915 | Apoptosis | 13 | 0.00173 | BP | GO:0005615 | Extracellular space | 13 | 6.0E-5 | CC | |
| GO:0006950 | Response to stress | 7 | 0.00173 | BP | GO:0005577 | Fibrinogen complex | 3 | 6.0E-5 | CC | |
| GO:0007050 | Cell cycle arrest | 6 | 0.00788 | BP | GO:0031093 | Platelet α granule lumen | 4 | 8.1E-4 | CC | |
| GO:0045944 | Positive regulation of transcription from RNA polymerase II promoter | 7 | 0.01021 | BP | ||||||
| GO:0045740 | Positive regulation of DNA replication | 3 | 0.01720 | BP | ||||||
| GO:0008360 | Regulation of cell shape | 4 | 0.02121 | BP | ||||||
| GO:0008285 | Negative regulation of cell proliferation | 8 | 0.02486 | BP | ||||||
| GO:0000122 | Negative regulation of transcription from RNA polymerase II promoter | 7 | 0.02486 | BP | ||||||
| GO:0009611 | Response to wounding | 3 | 0.02698 | BP | ||||||
| GO:0030183 | B cell differentiation | 3 | 0.02698 | BP | ||||||
| GO:0007229 | Integrin-mediated signaling pathway | 5 | 0.02698 | BP | ||||||
| GO:0006954 | Inflammatory response | 7 | 0.02698 | BP | ||||||
| GO:0007179 | Transforming growth factor β receptor signaling pathway | 4 | 0.02698 | BP | ||||||
| GO:0043066 | Negative regulation of apoptosis | 4 | 0.02698 | BP | ||||||
| GO:0006935 | Chemotaxis | 5 | 0.04499 | BP | ||||||
| GO:0007010 | Cytoskeleton organization and biogenesis | 5 | 0.04841 | BP | ||||||
| GO:0006955 | Immune response | 10 | 0.04843 | BP | ||||||
| GO:0005576 | Extra cellular region | 31 | 9.0E-5 | CC | ||||||
| GO:0005615 | Extra cellular space | 14 | 6.6E-4 | CC | ||||||
| GO:0005622 | Intracellular | 29 | 0.00843 | CC | ||||||
| GO:0005737 | Cytoplasm | 40 | 0.03660 | CC | ||||||
ONTO-TOOLS/OE2GO was used to identify the differentially expressed GO terms based on the hypergeometric distribution and corrected P value (< 0.05). The GO identified number (GOID), GO term name (GO name), the number of genes changed within each functional gene category, P values are listed. GO terms with at least 3 genes changed and corrected P values < 0.05 are listed in Table 4.