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Apoptosis assay of melanoma cells after pharmacological PPARβ activation. TdT-dUTP terminal nick-end labelling (TUNEL) labelling of A375 melanoma cells was performed after 24 h of treatment with either GW0742 or GW501516. Nuclei were counterstained with DAPI (a). Cells in ten random fields were counted and the percentage of TUNEL-positive cells determined (b, c; n = 4)
