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. Author manuscript; available in PMC: 2010 Mar 20.
Published in final edited form as: Biochem J. 2009 Apr 15;419(2):437–445. doi: 10.1042/BJ20082003

Figure 1. Analysis of subcellular localization of Drosophila dPrx5 protein.

Figure 1

(A) The N-terminal part of the deduced amino acid sequence of dPrx5 protein. The predicted mitochondrial targeting sequence is underlined and the putative second start site is bold. (B) Immunoblot analysis of protein lysates extracted from nuclear (N), mitochondrial (Mt) and cytosolic (Cyt) fractions prepared from flies, S2 cells and S2 cells overexpressing dPrx5 tagged with V5/His6 epitopes (pIB-dPrx5 transfectant). Signals were obtained with anti-dPrx5 antibodies and with anti-aconitase (mitochondrial protein), anti-HDAC (nuclear protein) and anti-GCLm (cytosolic protein) antibodies to control for a specificity of subcellular fractions. Staining with Coomassie Blue (lower panels) was performed to control for a loading.