Skip to main content
. Author manuscript; available in PMC: 2010 Mar 20.
Published in final edited form as: Biochem J. 2009 Apr 15;419(2):437–445. doi: 10.1042/BJ20082003

Figure 7. The effects of dPrx5 overexpression on aging and resistance to oxidative stress.

Figure 7

(A) Immunoblot analysis of dPrx5 overexpression. Subcellular fractions (upper panel) were prepared as described in Figure 1. Lower panel, samples were prepared from whole flies. Lanes 1, 2, 3 and 4, driver (Tub-GAL4/+) and three different transgene (UAS-dPrx5/+) controls; lanes 5, 6, and 7, three different experimental (UAS-dPrx5/Tub-GAL4) lines. (B) Life spans of flies overexpressing dPrx5 globally at low levels (with Arm-GAL4 driver) and at high levels (with Tub-GAL4 driver). Pooled data of two independent experiments are shown for each line. The median ages were extended in dPrx5/Arm experimental flies by 24.7 ± 3.7% compared with driver and by 13.4 ± 3.9% compared with transgenic controls. With Tub-GAL4 driver, median age was extended by 29.2 ± 7.7% compared with driver and by 30.0 ± 4.6% compared with transgenic controls. (C) Resistance of 30-day-old flies to 2.5 mM paraquat. Approx. 100–150 flies of each group were fed 1% sucrose solution containing 5 mM paraquat. Similar results were obtained in two independent experiments. The median survival time in experimental flies was increased by 52.6% to 60.0% compared with driver control and by 41.5% to 52.3% compared with transgene controls.