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Synergistic effect of high magnitude cyclic stretch and VEGF on ROS production by pulmonary endothelial cells. Static controls or cells preconditioned at 18% CS were exposed to vehicle or VEGF (200 ng/ml, 15 min), and ROS production was measured using EC preincubation with fluorescent ROS sensor DCFDA followed by fluorimetric analysis. Shown are mean ± SD of three independent experiments, *p < 0.05.
