FIG. 4.

Lack of mitochondria clustering in motor axons from rats overexpressing wild-type human SOD1. Motor axons were microdissected from 65-day-old rats overexpressing wild-type human SOD1 and stained to detect the human SOD1 protein (green) or CVIP mitochondrial protein (red). (A1–4) The signal obtained from a single confocal section of motor axons, whereas (B1–4) show a projection of multiple Z optical sections of several motor axons. Note the low degree of colocalization in (A4) and (B4), even when several optical sections were projected in the same plane (B4). Scale bar, 100 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article at www.liebertonline.com/ars).