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. 2009 Jul;11(7):1535–1545. doi: 10.1089/ars.2009.2614

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Copyright 2009, Mary Ann Liebert, Inc.

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FIG. 8. — Cytochrome c and ubiquitin immunoreactivity in motor axons from SOD1-G93A rats. (A) Cytochrome c (red) labelling followed the pattern of mitochondria distribution. Note the diffuse pattern of staining surrounding the mitochondria clusters, suggesting release of the protein due to organelle damage. (B) Comparison of ubiquitin immunoreactivity between 65-day-old axons (B) and 100-day-old axons (B1). In both cases, the image was obtained by merging DIC and confocal images. Bar, 10 μm. Note that ubiquitin staining in non-transgenic motor axons is comparable to that in 65-day-old SOD1-G93A rats. (C) Ubiquitin (green) was detected together with mitochondria (red) in a motor axon from a 110-day-old SOD1-G93A rat. Scale bars (B) and (C), 10 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article at www.liebertonline.com/ars).