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. 1997 Dec 9;94(25):14031–14036. doi: 10.1073/pnas.94.25.14031

Figure 2.

Figure 2

Immunoblots of PS-1 in extracts from several cultured cells. All lanes 1 are of PS-1-transfected DAMI cells; all lanes 2, Chinese hamster ovary cells; all lanes 3, IMR-32 cells; all lanes 4, IMR-90 cells; all lanes 5, NT2 cells; and all lanes 6, Cos-1 cells. (A) Extracts were prepared by Method A-Cells, labeled with antibody L3. (B) Prepared by Method B-Cells, labeled with antibody L3. (C) Prepared by Method B-Cells, labeled with antibody I1. In B, antibody L3-labeled CTF endoproteolysis products (brackets); in C, antibody I1-labeled NTF endoproteolysis products (brackets and lower of the two arrowheads). The single arrowheads in A and B and the upper arrowhead in C denote the intact monomeric PS-1 band.