Table 1.
Measured and predicted properties of H. beehleri GFP compared with representative fluorescent proteins from the three other known FP-bearing phyla. Species were chosen as the first-discovered or most commonly encountered representative of that taxonomic group. Length is in amino acids, charge is the hypothetical charge based on the primary sequence. Molecular weights in kilodaltons. Calculated values were determined using the protcalc.pl script, available at http://www.mbari.org/~haddock/scripts/.
name | taxon | length | charge | mol wt | accession number |
---|---|---|---|---|---|
Haeckelia GFP | Ctenophora | 262 | −9 | 30.3 | GQ903692 |
Aequorea GFP | Hydromedusa | 238 | −5 | 26.9 | M62653 |
Anthomedusa GFP | Hydromedusa | 225 | 4 | 25.0 | AY485334 |
Discosoma RFP | Corallimorph, Hexacoral | 225 | 1 | 25.8 | AF168419 |
Corynactis YFP | Corallimorph, Hexacoral | 221 | 0 | 25.0 | AY823227 |
Renilla GFP | sea pansy, Octocoral | 222 | −4 | 24.8 | AF372525 |
Cerianthus OFP | tube anemone, Anthozoa | 222 | −1 | 25.1 | AF545830 |
Pontella GFP | Copepoda, Crustacea | 221 | −2 | 24.9 | AY268074 |
Branchiostoma GFP | lancelet, Chordata | 223 | −2 | 23.7 | EF157660 |