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ELISpot analysis. The percentage of T cells secreting IFN-γ in response to FSP11-loaded target cells was determined in a series of ELISpot experiments. An exemplary sample is shown. Reactivity against unloaded, irrelevant MP-peptide-loaded and FSP11-loaded CD40 Bs is displayed. Analysis was performed in triplicates with 1000 effector cells per well.
