Table 2.

Results of cancer chemopreventative and anticancer assays performed with fractions from the extract of the sponge sponge Suberea sp.

Fract. No.	NFκB % Inhib a	NFκB % Survival b	NFκB IC50 c	DPPH % Inhib d	DPPH IC50 (ug/mL) e	MTT (HL-60 leukemia cells) % Survivalf	MTT IC50 (ug/mL) g	RXR Fold induction h	QR IR i	QR % Survival j	Aromatase % Inhib k	Aromatase IC50 (ug/mL)l	[NO] % Inhib m	[NO] % Survival n	SRB (MCF-7) % Survivalo	SRB (KB) % Survival p
1	81.7	55.0	13.8	21.9		102.9		1.1	0.9	100.0	19.90		12.25	155.25	89.2	75.0
2	78.9	77.6	16.0	26.1		82.3		1.1	1.0	100.0	46.73		15.80	143.05	93.5	72.1
3	31.7	67.3	8.9	17.2		136.3		1.3	1.5	100.0	68.75		64.45	155.95	72.7	70.0
4	68.7	62.1	2.6	19.4		103.9		1.2	0.8	100.0	23.85		15.90	134.00	91.3	75.5
5	43.1	76.9	4.2	74.7	155.2	98.4		1.3	2.2	36.4	71.77	13.32	37.10	149.15	62.8	71.9
6	31.3	47.6		61.0	181.3	-31.5	7.1	1.2	1.8	42.0	67.52		48.00	137.85	70.6	72.0
7	60.4	90.2		37.7		53.7		1.2	1.8	100.0	70.54	13.8	60.60	148.65	72.3	79.6
8	-7.8	12.0		36.3		76.3		1.0	2.0	100.0	75.71	6.16	46.10	143.85	62.0	71.2
9	-17.5	28.2		18.4		64.4		1.2	2.0	100.0	83.87	1.89	78.20	145.95	85.6	73.7
10	9.0	3.5		10.4		54.9		1.2	2.3	100.0	89.89	0.56	89.70	134.60	70.2	65.5
11	55.3	13.7		4.8		124.4		1.0	3.5	69.7	87.23	2.89	82.65	131.05	75.4	56.9
12	66.4	16.8		5.8		91.9		1.1	1.0	100.0	25.06		15.95	125.10	84.5	73.8
13	5.8	39.0		3.7		-20.4	19.3	1.2	1.1	100.0	82.76	7.45	40.10	130.15	87.8	64.0
14	14.6	56.8		5.0		93.8		1.3	1.1	100.0	89.03	0.74	89.25	129.75	68.1	71.1
15	5.3	33.6		11.2		78.0		1.3	2.2	100.0	77.13	1.73	81.95	129.60	50.6	60.8
16	-18.7	43.6		7.6		91.9		1.2	1.0	100.0	66.41		27.60	121.80	72.4	74.4

Test concentrations in each of the assays: NFkB: 20 ug/mL; DPPH: 200 ug/mL; MTT with HL-60 leukemia cells: 20 ug/mL; RXR: 20 ug/mL; QR: 20 ug/mL; Aromatase: 20 ug/mL; NO: 20 ug/mL; Cytotoxicity SRB assays: 20 ug/mL

^a100 % activity is with TNF as activator

^bTo avoid false positives and to determine cytotoxic effect on NFkB 293/Luc cells, 100% is with TNF and DMSO

^cSample concentration which caused 50% inhibition of NFkB

^d100% activity is with scavenging DPPH free radical

^eSample concentration which caused 50% inhibition of DPPH

^f100% survival is with DMSO

^gSample concentration which caused 50% inhibition in HL-60 cell survival

^hOne (1.0) fold is with control induction level of RXR transcriptional activity

ⁱQR the induction ratio (IR) of QR represents the specific enzyme activity of agent-treated cells compared with DMSO-treated control

^j100% survival is with DMSO

^k100% activity is with DMSO

^lSample concentration which caused 50% inhibition of aromatase

^m100% activity is with LPS as activator

ⁿTo avoid false positives and to determine cytotoxic effect on RAW 264.7 cells, 100% is with LPS and DMSO

^o100% survival is with DMSO

^p100% survival is with DMSO