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. Author manuscript; available in PMC: 2010 Mar 22.
Published in final edited form as: Science. 2010 Jan 15;327(5963):340–343. doi: 10.1126/science.1174779

Fig. 4. The role of Gα13 in clot retraction and dynamic RhoA regulation.

Fig. 4

(A) Effect of 250 μM mSRI peptide on clot retraction of human platelet-rich plasma compared with DMSO and scrambled peptide. Clot sizes were quantified using Image J (mean±SD, n=3, t-test). (B) Comparison of clot retraction (mean±SD, n=3, t-test) mediated by control siRNA platelets and Gα13-depleted platelets. (C, D, E, F) Platelets were stimulated with thrombin with or without 2 mM RGDS, and monitored for turbidity changes of platelet suspension caused by shape change and aggregation (C). The platelets were then solubilized at indicated time points and analyzed for amount of β3 coimmunoprecipitated with Gα13 (D) and amount of GTP-RhoA bound to GSTRBD beads (E) by immunoblot. (F) quantitative data (mean±SD) from 3 experiments. (G) A schematic illustrating Gα13-dependent dynamic regulation of RhoA and crosstalk between GPCR and integrin signaling.