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. Author manuscript; available in PMC: 2010 Mar 22.
Published in final edited form as: J Immunol. 2007 Apr 1;178(7):4473–4481. doi: 10.4049/jimmunol.178.7.4473

FIGURE 5.

FIGURE 5

gpUL18 NK inhibition is mediated via the LIR-1 receptor. Autologous CD107a mobilization assays were set up with IFN-α-activated PBMC against primary skin fibroblasts from donor 3. Fibroblasts were infected for 72 h with RAd vectors containing the UL18 ORF (RAd-UL18) or with the empty control adenovirus (RAd-Ctrl) (500 PFU/cell). A saturating amount (20 μg/ml) of anti-LIR-1 F(ab’)2 or control anti-CD56 mAb was added on PBMC for 15 min at 37°C before contact with targets and throughout the assays (***, p < 0.001 with two-way ANOVA analysis assuming not-repeated measures followed by Bonferroni post tests). Results are means ± SEM of duplicate samples and are representative of three independent experiments.