FIGURE 6.

Cross-linking of GPI-anchored CD59, whether glycosylated or unglycosylated, triggers intracellular Ca²⁺ concentration increase in U937. CD59 (A) and CD59 mutants (B) were either incorporated into U937 or stably expressed in this line. Cells were subjected to Ab-mediated cross-linking, indicated by the arrow, and intracellular Ca²⁺ concentration levels were determined by measuring the spectral shift of preloaded fura 2-Ca²⁺ indicator. Results are representative of three independent experiments.