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. Author manuscript; available in PMC: 2010 Mar 22.
Published in final edited form as: J Immunol Methods. 2005 Oct 21;307(1-2):24–33. doi: 10.1016/j.jim.2005.08.015

Fig. 5.

Fig. 5

Comparison of cytotoxicity of NK clones cultured with irradiated, allogeneic PBMC feeders derived from different numbers of donors. NK clones were isolated using single cell sorting direct and cultured in SCGM supplemented with 5% AB serum and IL-2 (500 IU/ml) and OKT3 (50 ng/ml). 5×104 irradiated allogeneic feeders from different numbers of donors were used for stimulation. Cytotoxic NK assays using K562s as targets were performed on day 17. Positive wells were identified as lysing targets at greater than 10%. The variation indicated by the bars represents frequency calculated using maximum and minimum control release minus 1 SD. χ2 tests showed significant differences between the indicated results at ap= 0.0082.