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. Author manuscript; available in PMC: 2010 Mar 22.
Published in final edited form as: J Neuroimmune Pharmacol. 2009 Sep 16;4(4):476–488. doi: 10.1007/s11481-009-9169-z

Table 1.

SMART 5′ RACE primers used to identify the TSS.

Primer namea Sequence 5′-to-3′ Size (mer) 5′ end binding siteb
Mouse GSP1 mCB2-R301 CGACCCCGTGGAAGACGTGGAAGATGACAA 30 301bp downstream ATG
Mouse GSP2 mCB2-R217 TGAACAGGTACGAGGGCTTTCT 22 217bp downstream ATG
Human GSP1 hCB2-R298 GCCAGGAAGTCAGCCCCAGCCAAGCTGCCAA 31 298bp downstream ATG
Human GSP2 hCB2-R163 GCACAGCCACGTTCTCCAGGGCACTTAGCA 30 163bp downstream ATG
a

GSP, gene specific primer; 1 designates used for the initial RACE PCR, 2 used for nested PCR.

b

The number of base pairs from the start of translation in which the 5′ end of the GSP binds for amplification of CB2. The primers were designed to include enough of the coding region for CB2 confirmation of the RACE transcripts.