Table 2.

Primers and taqman probes used in this study.

Primer pairsa and probe		Sequence 5′-to-3′b	Assayc	Size of amplicond
mCB2-E3	F	GCCGTGCTCTATATTATCCTGTCCTC	qRT-PCR	120 bp
	R	GACAAAGTTGCAGGCGAAGATCAC
	P	6FAM-AGAAAGCCCTCGTACCTGTTCATCAGCA-BHQ1
mCB2-E1a	F	TCATCTGCGAAAGTGTGA	qRT-PCR	112 bp
	R	TTGTCCTGGCTATTCTGTATC
	P	6FAM-CTGGAGCTGCAGCTCTTGGGAC-BHQ1
mCB2-E1b	F	ACACATAGCCTGGCACA	qRT-PCR	171 bp
	R	GCGGTTGAATTCTCTCTTC
	P	6FAM-TCAAGTGAGTTGCAGGACAGCATAC-BHQ1
mCB2-E2	F	TTCTAGAAGGCACCCATGT	qRT-PCR	189 bp
	R	CCTCTGCTCATTCAGGTACA
	P	6FAM-CTTCCTGTTGCTGTGTGCATCCT-BHQ1
β-actin	F	GGGAATGGGTCAGAAGGACT	qRT-PCR	134 bp
	R	AGGTGTGGTGCCAGATCTTC
	P	ROX-ATGTGGGTGACGAGGCCCAGAGCAA-BHQ2
mE1b-DNA	A	ggaggaggcatgaggca	PCR	189 bp
mRNA	B	ACACATAGCCTGGCACA	RT-PCR	171 bp
	C	GCGGTTGAATTCTCTCTTC
	G	GACAAAGTTGCAGGCGAAGATCAC		755 bp
mE2-DNA	D	atacatcaaacacatccttg	PCR	224 bp
mRNA	E	TTCTAGAAGGCACCCATGT	RT-PCR	189 bp
	F	CCTCTGCTCATTCAGGTACA
	G	GACAAAGTTGCAGGCGAAGATCAC		567 bp
hE1-DNA	J	gcaagagaaagctggctt	PCR	99 bp
mRNA	I	TCAACAGGTGCTCTGAGTG	RT-PCR	71 bp
	H	CTGAGGAGTCCCAGTTGTT

^aName of primers; m, mouse; h, human; E3, exon 3; E1a, exon 1a; E1b, exon 1b; E2, exon 2; F, forward primer; R, reverse primer; P, Taqman® probe; A, D, J, DNA forward primers; B, E, I, forward primers for amplification of mRNA derived cDNA; C, F, G, H, reverse primers.

^bSequences of the primers and probes. 6FAM, 6-carboxyfluorescein; BHQ1 or 2, Black Hole Quencher®-1 or 2. Primers designed to bind genomic DNA 5′ of the TSSs are in lower case.

^cThe assay the primers were used in. qRT-PCR, quantitative real time, reverse transcription polymerase chain reaction.

^dSize of the expected amplicons.