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. Author manuscript; available in PMC: 2010 Mar 22.
Published in final edited form as: J Immunol. 2009 Feb 1;182(3):1278–1286. doi: 10.4049/jimmunol.182.3.1278

Figure 3. Association of Treml4 with DAP12.

Figure 3

A, Amino acid sequence alignment of human and mouse Treml4. Human (GenBank accession number, NM198153) and mouse Treml4 share an overall amino-acid identity of 39.9%. The predicted signal peptide (mouse, residues 1–30; human, 1–23) and transmembrane segments (TM; mouse, 200–333) are indicated. B, Real-time RCR analysis of Treml4 mRNA. Values are relative to expression of the gene encoding GAPDH. BMCs, bone marrow cells; LNs, lymph nodes. C, HEK293 cells were transiently transfected with the indicated combination of Treml4-FLAG, myc-FcRγ, myc-DAP10, and myc-DAP12 (above lane). After 36 hours, cell lysates were immunopreciptated (IP) with anti-FLAG antibody, followed by blotting (IB) with anti-myc antibody. WCL, whole cell lysate; n.s., non-specific bands. D, Thymocytes were exposed to γ-irradiation to induce apoptosis and then stained with the indicated chimeric fusion proteins. E, Thymocytes were incubated at 56°C for 30 min and then stained with fusion proteins and analyzed by FACS. Representative data from at least two independent experiments were shown.