A, Parental CHO cells or Treml4 stable transfectants were stained with anti-Treml4 monoclonal antibody. B, Splenocytes or lymph node cells were prepared with collagenase digestion, and the cells were stained with control antibody (open histogram) and anti-Treml4 antibody (gray histogram). The expression of Treml4 was monitored by FACS on B220+ B and CD3ε+ T cells, CD8α+ and CD8α− DCs (CD8α+ CD11c+ and CD8α−CD11c+, respectively) and B220+ plasmacytoid DCs (B220+ CD11c+).